Extended Data Fig. 7: Blocking ND astrocyte BMP signaling reduces neural deficits.
a,b. BMP6-treated WT astrocytes show protein secretion (a) and gene expression (b) downregulations that overlap with ND astrocytes. N = 6 cultures WT, FXS, RTT, DS, plus 6 cultures WT + /- BMP6 proteomics; N = 6 cultures WT, FXS, RTT; 4 DS, plus 6 cultures WT + /- BMP6 RNA sequencing. c. Example images from Fig. 7d prior to processing and analysis. Neurons immunostained with MAP2 (dendrites, green) and tau (axon, red). d. Relative frequency distribution plot of total neurite outgrowth length, same data as Fig. 7e. Data from 3 experiments, number of neurons: alone=467, WT ACM = 733, BMP6 WT ACM = 610.e. Relative frequency distribution plot of total neurite outgrowth length, same data as Fig. 7 f. Data from 3 experiments, number of neurons: alone=378, WT ACM = 380, BMP6 WT ACM = 506, BMP6 WT ACM + Igfbp2 blocking Ab=335. f. Example images from Fig. 7h prior to processing and analysis. Neurons immunostained with MAP2 (dendrites, green) and tau (axon, red). g. Relative frequency distribution plot of total neurite outgrowth, same data as Fig. 7i. Data from 3 experiments, number of neurons: alone=923, WT ACM = 1164, FXS ACM = 1132, Noggin FXS ACM = 1099. h. Relative frequency distribution plot of total neurite outgrowth, same data as Fig. 7j. Data from 3 experiments, number of neurons: alone=238, WT ACM = 279, RTT ACM = 387, Noggin RTT ACM = 365. i. Example images of cortical neurons treated with noggin at the time of plating, ± WT ACM or ± FXS ACM (image merge of MAP2 + Tau). j. Quantification of total neurite outgrowth, data from 3 experiments. Number of neurons: alone=2197, alone + noggin=1981, WT ACM = 2167, WT ACM + noggin=2421, FXS ACM = 2060, FXS ACM + noggin=2523. Violin plots dashed line marks median, dotted lines 25th and 75th percentile. Statistics by Kruskal-Wallis one-way ANOVA on ranks with Dunn’s test for multiple comparisons.
