Extended Data Fig. 4: sgRNA abundance distribution in CRISPRi screens, comparison of phenotypes from VCAM1 and phagocytosis CRISPRi screens, and validation of selected hits from CRISPRi screens.
a, sgRNA abundance distribution for the CRISPRi screens shown in Fig. 3. b, Gene scores (see Methods) from the phagocytosis vs. VCAM1 CRISPRi screen against transcription factors (left) or the druggable genome (right) in iAstrocytes treated with IL-1α+TNF+ C1q. c-d, Phagocytosis of pHrodo-labeled synaptosomes (c) or induction of cell-surface VCAM1 (d) by iAstrocytes transduced with non-targeting sgRNA (NTC) vs. sgRNAs targeting selected hits from the screens shown in Fig. 3, treated with vehicle control or IL-1α+TNF+ C1q (n = 6 wells for NTC, n = 3 wells for knockdowns). MFI: median fluorescence intensity measured by flow cytometry. In panels c and d, P values were calculated by linear regression (two-sided Wald test; see Methods) and adjusted for multiple testing (Padj; Holm’s method) per family of tests (all comparisons within a plot).
