Extended Data Fig. 4: Reduction of mGluR1a signals after HOKR training.
From: Synaptic pruning through glial synapse engulfment upon motor learning
a, Representative image of mGluR1a punctate signals in the molecular layer (ML) of the cerebellar cortex. b, High magnified images from Fig. 4f of the mGluR1a punctate signals localized on PC spines (Arrowheads: spine-like localization). VGLUT1 positive parallel fiber boutons appose these mGluR1a puncta. Arrowheads: spine-like localization. We have done more than 4 independent experiments. c, Cumulative frequencies of distal ML of mGluR1a IHC signal intensity. Significant reduction of the mGluR1a intensity was observed in HOKR trained mice (n = 6 mice). Kolmogorov-Smirnov test (**** P < 0.0001). d, The reduction of the mGluR1a immunostaining signal intensity after HOKR training was not dependent on the depth from the slice surface (n = 3 mice). Two-sided unpaired t-test (* p = 0.05, 0.037, 0.024, 0.023, 0.016, 0.011, 0.0012, 0.014 from 0 to 2.31 µm with 0.33 µm step). e, The intensity of mGluR1a in Pfl was not different between sham and HOKR trained mice (n = 6 mice). Two-sided unpaired t-test (p = 0.88). Data are shown mean ± s.e.m.
