Fig. 1: A high-molecular-weight tau seed interacts with BSN protein.
From: Bassoon contributes to tau-seed propagation and neurotoxicity
a, Schematic of the tau-seeding assay of SEC fractions. b, Tau-seeding activity of SEC fractions obtained from 3-month-old PS19 and WT mouse brain lysates. c, Total human tau (hTau) detected by ELISA in SEC fractions from PS19 and WT mouse brain lysates. d, Tau-seeding activity of the PS19 SEC fraction 9 (F9) containing HMW tau, before and after hTau IP using HT7 antibody. e, EM of the F9 IP product showing short tau filaments, and width distribution of these filaments. Scale bar, 100 nm. The experiment was repeated three times with similar results. f,g, Volcano plot indicating tau interactors found in F9 (f) and F17 (g), identified by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). h, Western blot of BSN from co-IP of human tau (HT7) from F9 in PS19 brain lysates. i, Representative immunofluorescence in PS19 mice cortex for pathological tau (PHF1) and BSN protein. Colocalized pixels are shown in white, whereas nuclei are visualized in cyan. The merged panel includes orthogonal images of reconstructed three-dimensional views. Colocalization analysis was performed to determine pixel intensity correlation between PHF1 and BSN. Scale bar, 25 µm. Data are shown as the mean ± s.e.m. Experiments were performed in triplicates (b–d, h and i), and significance was determined by an unpaired two-tailed Student’s t-test (d). CFP, cyan fluorescent protein; YFP, yellow fluorescent protein.
