Extended Data Fig. 9: Bassoon downregulation improves behavioral and electrophysiological impairments in PS19 males and females.
From: Bassoon contributes to tau-seed propagation and neurotoxicity
a, b, Average of fEPSP slope from minute 60 to 70 by sex (a) and paired-pulse ratio (PPR) (b), in 6-month-old WT and PS19 mice injected with shBSN or scramble shRNA. c-f, 2-paw test (c), 4-paw test (d), body temperature (e), and frailty test (f), in 9-month-old WT and PS19 mice injected with shBSN or scramble shRNA. g-j, Quantification of MC1 immunostaining as a percentage of area (g), pTauS396/S404 (PHF1) protein levels (h), pTau Thr231 protein levels (i), and total human tau levels by ELISA (j), in shBSN and scrambled PS19 male and female mouse brain lysates. Data represent the mean ± s.e.m. Experiments were performed with n = 7 for males and n = 4 for females for WTscramble, n = 4 for males and n = 6 for females for PS19scramble, n = 7 for males and n = 4 for females for WTshBSN, and n = 6 for males and n = 5 for females for PS19shBSN mice (a), n = 5 for males and n = 6 for females for WTscramble, n = 5 for males and n = 5 for females for PS19scramble, n = 5 for males and n = 5 for females for WTshBSN, and n = 5 for males and n = 6 for females for PS19shBSN mice (b), n = 10 for males and n = 10 for females for WTscramble, n = 9 for males and n = 12 for females for PS19scramble, n = 13 for males and n = 9 for females for WTshBSN, and n = 8 for males and n = 8 for females for PS19shBSN mice (c-f), and n = 4 per sex (g-j). Significance was determined by one-way ANOVA (a–f), and unpaired two-tailed Student’s t-test (g–j).
