Fig. 4: Bassoon contributes to tau spreading in vivo.
From: Bassoon contributes to tau-seed propagation and neurotoxicity
a, Schematic of the GFP-P2A-hTauP301L tau-spreading mouse model. GFP+ neurons are hTau donors, spreading hTauP301L to recipient GFP− neurons. b, Timeline of injections in WT mice. Animals were injected with scramble or shBSN AAV at P0, then with AAV-GFP-P2A-hTauP301L at P90. Animals were euthanized at P180 to evaluate hTau spreading. c, Representative immunofluorescences for hTau (HT7 antibody) in animals overexpressing GFP-P2A-hTauP301L injected with scramble shRNA and BSN shRNA. Scale bar, 100 µm. d, Quantification of cortical hTau+/GFP− cells (top) and GFP+ cells (bottom) per mm2. For box plots, the center line represents the median, boundaries denote the interquartile range, and the whiskers represent the lower and upper limits. Values are given as the means ± s.e.m. Experiments were performed with n = 4, and significance was determined by unpaired two-tailed Student’s t-test.
