Extended Data Fig. 2: Bassoon is associated with tau pathology in PS19 mice.
From: Bassoon contributes to tau-seed propagation and neurotoxicity
a, Representative immunofluorescence and colocalization for BSN (red), PHF1 (green), nuclei (cyan), and merge (yellow), in 3-, 6- and 9-month-old wild-type and PS19 mouse cortexes. Scale bar: 200 µm for main images and 50 µm for insets. Merge image for PS19 includes orthogonal image of reconstructed three-dimensional views. Colocalization analysis was performed to determine pixel intensity correlation between PHF1 and BSN. b, Pearson’s correlation of BSN and PHF1 intensity in 3-, 6- and 9-month-old wild-type and PS19 mouse cortexes. c, 3-month-old PS19 mouse sections immunostained against PHF1, BSN, and Syn-1 (upper panels), and colocalization of PHF1 and BSN (colored yellow; lower left panel). PHF1/BSN colocalization was re-colocalized with the Syn-1 channel to obtain a final triple-colocalization between PHF1/BSN and Syn-1 (lower middle and right panels). d, Percentage of colocalization between PHF1/BSN puncta and Syn-1. Data are the mean ± s.e.m. Experiments were performed with n = 3 (a–d). Significance was determined by one-way ANOVA (b).
