Extended Data Fig. 10: Analysis of protein biosynthesis in vitro and in vivo after lipoamide treatment.
a, Scheme of microglial cell line preparation for polysome profiling. b, Polysome profiles of differently treated microglial cell lines. c, Dot plot depicting the polysome/monosome ratio in microglial cell lines, treated with different concentrations of DMSO or lipoamide, or harringtonine as a control. Data are shown as mean ± SEM. n = 4 per group. Each point represents one replicate. Significance was calculated with Brown-Forsythe and Welch ANOVA tests, with post-hoc Dunnett’s test for multiple comparisons. d, Scheme of cerebroventricular injections of OPP and CHX in DMSO-treated or lipoamide-treated brains, followed by FACS analysis. Abbreviations: OPP = O-propargyl-puromycin, CHX = cycloheximide. e, FACS plots depicting average intensity of the signal in OPP+ cells in different conditions in vivo, indicating actively translating cells in the adult zebrafish telencephalon. f, Dot plot of OPP+ intensity in DMSO- and lipoamide-treated brains. Data are shown as mean ± SEM. n = 5 per group. Each point represents one animal. Significance was calculated with unpaired Student’s t-test.
