Extended Data Fig. 2: Dynamics of Extracellular 5-HT Level in BA of Female and Male Mice under Social and Anxiogenic Stimuli.
From: Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety
(a, b) Average traces (a, mean ± 95% CI; dash line: start of social interaction) and statistics (b, GFP, n = 5 mice; GRAB5-HT2h, n = 6 mice; two-sided RM two-way ANOVA followed by Holm–Sidak correction, F(1, 9) = 62.69, P < 0.0001; GRAB5-HT2h Baseline versus GRAB5-HT2h Social, ****P < 0.0001; GFP Social versus GRAB5-HT2h Social, ****P < 0.0001) of GFP and GRAB5-HT2h fluorescence under social stimulus in female mice. (c, d) Average traces (c, mean ± 95% CI; dash line: start of ~300 Lux stimulus) and statistics (d, GFP, n = 5 mice; GRAB5-HT2h, n = 6 mice; two-sided RM two-way ANOVA followed by Holm–Sidak correction, F(1, 9) = 23.98, P = 0.0009; GRAB5-HT2h 20 versus 300 Lux, ****P < 0.0001; 300 Lux, GFP versus GRAB5-HT2h, ****P < 0.0001) of GFP and GRAB5-HT2h fluorescence under ~300 Lux stimulus in female mice. (e, f) Average traces (e, mean ± 95% CI; dash line: start of social interaction) and statistics (f, mean ± SEM; n = 6 female and 7 male mice; two-sided unpaired t test; t(11) = 0.2916, P = 0.7760) of GRAB5-HT2h fluorescence in male and female mice under social stimulus. (g, h) Average traces (g, mean ± 95% CI; dash line: start of ~300 Lux stimulus) and statistics (h, mean ± SEM; n = 6 female and 6 male mice; two-sided unpaired t test; t(10) = 0.5323, P = 0.6062) of GRAB5-HT2h fluorescence in male and female mice under ~300 Lux stimulus.
