Extended Data Fig. 8: DRN5-HT Neurons Inhibit a Subset of BAPyr Neurons via HTR1A but not GABA.
From: Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety
(a) Schematic of virus injection and electrophysiological recordings in BAPyr neurons under 5-ms light pulse stimulation of serotonergic terminals from DRN. (b) Representative trace of BAPyr neurons using a high chloride internal solution under 5-ms light pulse in the presence of TTX + 4AP. (c) Schematic of virus injection and electrophysiological recordings in BAPyr neurons under 55-Hz light train stimulation of serotonergic terminals from DRN. (d) Proportion of neurons with (responsive) or without (non-responsive) slow oIPSCs in BAPyr neurons during 55-Hz light train. (e) Diagrams of distribution of responsive (solid circles) and non-responsive (hollow circles) BAPyr neurons. (f) Representative traces (left, shadow: opto-activation) and statistics (right; n = 9 neurons from 3 mice; two-sided RM one-way ANOVA; F(8,16) = 27.17, P < 0.0001; ACSF versus CGP54626, t(8) = 1.132, P = 0.2904; CGP54626 versus WAY100635, t(8) = 5.815, **P = 0.0012; CGP54626 versus WAY100635, t(8) = 5.495, **P = 0.0012) of oIPSCs before and after application of CGP54626 (GABAB receptor antagonist, 1 μM) and WAY100635. (g) Representative traces (left, shadow: opto-activation) statistics (right; n = 6 neurons from 4 mice; two-sided paired t test; t(5) = 1.772, P = 0.1366) of oIPSCs before and after application of NAS-181 (1 μM).
