Extended Data Fig. 9: Activation of Serotonergic Terminals in BA Modulates Glutamate Transmission to HTR1A− Pyr Neurons, but not PV or HTR1A+ Neurons, via HTR1B.
From: Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety
(a-c) Schematic (a), representative traces (b) and statistics (c, mean ± SEM and individual data; n = 21 neurons from 4 mice; two-sided RM one-way ANOVA, F(20, 180) = 9.516, P < 0.0001) of the amplitude of oEPSCs in BAPV neurons that showed glutamatergic responses (R-BAPV neurons) in response to opto-stimulation. (d-f) Schematic (d), representative traces (e), cumulative frequency curves of inter-spike-interval (f, outside), and statistics (f, inside) of frequency (f, left; t(16) = 0.9465, P = 0.3580) and amplitude (f, right; t(16) = 0.6886, P = 0.5009) of sEPSCs in BAPV neurons that did not show glutamatergic responses (NR-BAPV neurons) before (light off) and after (light on) opto-stimulation (n = 17 neurons from 4 mice, two-sided paired t test). (g-i) Schematic (g), representative traces (h), cumulative frequency curves of inter-spike-interval (i, outside), and statistics (i, inside) of frequency (i, left; t(11) = 0.2341, NS P = 0.8192) and amplitude (i, right; t(11) = 1.166, NS P = 0.8192) of sEPSCs in HTR1A+ BAPyr neurons before (light off) and after (light on) opto-stimulation (n = 12 neurons from 4 mice, two-sided paired t test). (j-l) Schematic (j), representative traces (k), cumulative frequency curves of inter-spike-interval (l, outside), and statistics (l, inside) of frequency (t(29) = 5.419, ****P < 0.0001) and amplitude (t(29) = 0.3166, P = 0.7538) of sEPSCs in BA HTR1A− neurons before and after opto-stimulation (n = 30 neurons from 4 mice; two-sided paired t test). (m, n) Representative traces (m), cumulative frequency curves of inter-spike-interval (n, outside), and statistics (n, inside) of frequency (t(28) = 0.9280; P = 0.3612) and amplitude (t(28) = 0.6915; P = 0.4950) of sEPSCs in BA HTR1A− neurons before and after opto-train stimulation with the presence of NAS-181 (1 μM) (n = 29 neurons from 4 mice, two-sided paired t test). Shadows in f, i, l, k: opto-activation.
