Extended Data Fig. 10: Pharmacological Inhibiting HTR1A in BA Induced Avoidance of Bright Spaces but not Social Avoidance, without Affecting Locomotion.
From: Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety
(a) Schematic paradigm of cannula implantation and drug administration. (b-f) Statistics (mean ± SEM; n = 13 mice, two-sided unpaired t test) of time in open arm (t(24) = 4.324; ***P = 0.0002) in EPM (b), time in centre (t(24) = 3.718; **P = 0.0011) in OFT (c), latency to feed (t(24) = 3.411; **P = 0.0023) in NSF (d) and interaction time (t(24) = 1.483; P = 0.1512) in social interaction test (e), sniffing time (F(3, 48) = 13.78, P < 0.0001; E versus S1, ACSF, t(48) = 11.39, ****P < 0.0001, WAY100635, t(48) = 9.254, ****P < 0.0001; S1 versus S2, ACSF, t(48) = 4.218, ****P = 0.0001, WAY100635, t(48) = 0.703, ****P < 0.0001; ACSF versus WAY100635, S1, t(96) = 2.551, *P = 0.0245, S2, t(96) = 0.3671, P = 0.7144), social index (F(1, 24)pharmacology = 0.0945, P = 0.7611; ACSF versus WAY100635, sociability, t(48) = 0.9231, P = 0.36.6, social novelty, t(48) = 1.347, P = 0.3347) in three-chamber test (f; n = 13 mice; two-sided RM two-way ANOVA) after administration of ACSF or WAY100635 in BA bilaterally in C57 mice. (g) Schematic paradigm of cannula implantation and drug administration. (h-i) Statistics (mean ± SEM) of total distance (F(2, 36) = 1.659; P = 0.2045) in OFT (h) and total food consumed (F(2, 36) = 0.196; P > 0.8229) in NSF (i) after administration of ACSF, WAY100635 and NAS-181 in BA bilaterally in C57 mice (n = 13 mice, two-sided RM one-way ANOVA).
