Extended Data Fig. 1: High Ambient Illumination Induces Anxiety-related Behaviours.

Representative traces and statistics (mean ± s.e.m) of time in open arm (F_{(2, 57)} = 11.13, P < 0.0001; 20–300 Lux, t₍₅₇₎ = 3.563, **P = 0.0015; 20–600 Lux, t₍₅₇₎ = 4.459, ***P = 0.0001; 300–600 Lux, t₍₅₇₎ = 0.8959, P = 0.3741) of EPM (a), time in centre (F_{(2, 57)} = 4.716, P = 0.0127; 20–300 Lux, t₍₅₇₎ = 2.760, *P = 0.0231; 20–600 Lux, t₍₅₇₎ = 2.547, *P = 0.0270; 300–600 Lux, t₍₅₇₎ = 0.2125, P = 0.8324) and total distance (F_{(2, 57)} = 0.6638, P = 0.5188) in OFT (b), latency to feed (F_{(2, 57)} = 22.45, P < 0.0001; 20–300 Lux, t₍₅₇₎ = 0.1996, P = 0.8425; 20–600 Lux, t₍₅₇₎ = 5.900, ****P < 0.0001; 300–600 Lux, t₍₅₇₎ = 5.701, ****P < 0.0001) and total food consumed (F_{(2, 57)} = 0.1877, P = 0.8294) in NSF (c), and interaction time (F_{(2, 57)} = 26.33, P < 0.0001; 20–300 Lux, t₍₅₇₎ = 3.247, **P = 0.002; 20–600 Lux, t₍₅₇₎ = 7.244, ****P < 0.0001; 300–600 Lux, t₍₅₇₎ = 0.3997, ***P = 0.0004) in social interaction test (d) (two-sided RM one-way ANOVA followed by Holm–Sidak correction), sniffing time (F_{(5, 114)} = 18.46, P < 0.0001; S1, 20–300 Lux, t₍₂₂₈₎ = 4.591, ****P < 0.0001, 20–600 Lux, t₍₂₂₈₎ = 8.918, ****P < 0.0001, 300–600 Lux, t₍₂₂₈₎ = 4.327, ***P = 0.0002; S2, 20–300 Lux, t₍₂₂₈₎ = 1.347, P = 0.5467, 20–600 Lux, t₍₂₂₈₎ = 5.765, ****P < 0.0001, 300–600 Lux, t₍₂₂₈₎ =4.419, ***P = 0.0001) and social index (F_{(2, 57)} = 1.358, P = 0.2653; F_{(2, 57) illumination} = 25.31, P < 0.0001; sociability, 20–300 Lux, t₍₁₁₄₎ = 2.632, **P = 0.0097; 20–600 Lux, t₍₁₁₄₎ = 5.568, ****P < 0.0001; 300–600 Lux, t₍₁₁₄₎ = 2.936, **P = 0.0080; social novelty, 20–300 Lux, t₍₁₁₄₎ = 0.4221, P = 0.6738; 20–600 Lux, t₍₁₁₄₎ = 3.393, ****P < 0.0001; 300–600 Lux, t₍₁₁₄₎ = 2.971, **P = 0.0072) in three-chamber test (e; two-sided RM two-way ANOVA followed by Holm–Sidak correction) (n = 20 mice).

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