Extended Data Fig. 6: In vivo imaging of Ca²⁺ dynamics from LH^Lepr neurons at different HFD stages.

(a, b) Experimental timeline and example traces of LH^Lepr neuronal activity from four representative cells during HFD priming stage. Blue dashed areas indicate eating bouts. (c) Raster plots showing LH^Lepr neuronal activity from two representative mice aligned to the first eating bout onset during HFD-priming. The rows and ticks in the raster plots represent individual cells and a single Ca²⁺ transient event, respectively. Blue dashed areas indicate eating bouts. (d) Pie charts indicate the percentage of LH^Lepr neurons generating Ca²⁺ transient events at the onset of eating bouts during HFD-priming stage (n = 36 cells from four mice). (e, f) Experimental timeline and example traces of LH^Lepr neuronal activity from four representative cells during the 1st Re-HFD. Blue dashed areas indicate eating bouts. (g) Raster plots showing LH^Lepr neuronal activity from two representative mice aligned to the first eating bout onset during the 1st Re-HFD. The rows and ticks in the raster plots represent individual cells and a single Ca²⁺ transient event, respectively. Blue dashed areas indicate eating bouts. (h) Pie charts indicate the percentage of LH^Lepr neurons generating Ca²⁺ transient events at the onset of eating bouts during the 1st Re-HFD stage (n = 43 cells from five mice). (i, j) Experimental timeline and example traces of LH^Lepr neuronal activity from four representative cells during the 4th Re-HFD. Blue dashed areas indicate eating bouts. (k) Raster plots showing LH^Lepr neuronal activity from two representative mice aligned to the first eating bout onset during the 4th Re-HFD. The rows and ticks in the raster plots represent individual cells and a single Ca²⁺ transient event, respectively. Blue dashed areas indicate eating bouts. (l) Pie charts indicate the percentage of LH^Lepr neurons generating Ca²⁺ transient events at the onset of eating bouts during the 4th Re-HFD stage (n = 43 cells from five mice).