Fig. 3: Fluoxetine manipulation alters neuromodulation in predicted SRNs.
From: Serotonin regulation of behavior via large-scale neuromodulation of serotonin receptor networks
a, ChR2 animals were treated with one pharmacologically significant dose of fluoxetine and then underwent ofMRI. b, Time-locked ofMRI amplitude changes of SRNs in ChR2 animals (left) and in ChR2 animals treated with fluoxetine (right). Each color represents an SRN. c, Results from permutation analysis of linear models testing for group differences in Htr1a, Htr1b and Htr4 SRN time-locked amplitude changes calculated via DR-stage 1, between the ChR2 group and the ChR2 group treated with fluoxetine. Statistical significance was assessed with 1,000 block-aware permutations (whilst allowing permutations only within-subject), with FWE-corr for multiple comparisons across time, SRNs and two tails. Showing −log10 FWE-corr P, corrected across time and two tails. Dashed lines demarcate statistical thresholds. These results show that known pharmacological effects on serotonin receptor types can be detected with our approach, hence providing an important validation, and that the spatial distribution of different serotonin receptor types confers functional specificity when selectively manipulating serotonin availability in synaptic terminals.
