Extended Data Fig. 1: Spatial correspondence between gene expression maps, SRNs, and PET maps.
From: Serotonin regulation of behavior via large-scale neuromodulation of serotonin receptor networks
Related to Fig. 1. a) Showing human brain maps (rank-based inverse normalised) of serotonin receptor genes publicly available from the Allen Brain Institute. b) Showing group-level maps (T-stats) of human brain SRNs functional connectivity (DR-stage 2) estimated by combining transcriptomic maps (displayed in panel a) with resting state fMRI data from the Human Connectome Project using FSL DR as exemplified in Fig. 1. c) Publicly available serotonin receptor density maps previously characterised using PET. (Note, only maps for receptors 5-HT1A, 5-HT2A, and 5-HT4, were available. Here maps are Z-scored for display purposes). d) Spatial PET—HTR correspondence between maps of serotonin receptor density previously characterised using PET (displayed in panel c) and human gene expression maps of serotonin receptors (displayed in panel a). Spatial similarity was calculated using Pearson’s correlation across atlas brain regions. Red squares highlight diagonal elements of the correlation matrix. e) Boxplot showing difference in PET—SRN correspondence between diagonal and off diagonal elements of panel d). f) Spatial PET—SRN correspondence between maps of serotonin receptor density previously characterised using PET (displayed in panel c) and human SRN functional connectivity maps (displayed in panel b). Spatial similarity was calculated using Pearson’s correlation across atlas brain regions. Red squares highlight diagonal elements of the correlation matrix. g) Boxplot showing difference in PET—SRN correspondence between diagonal and off diagonal elements of panel d). In e) and g), on each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers. Each data point is plotted individually (n = 9 maps). Together panels e) and g) show greater PET—HTR and PET—SRN spatial correspondence for the same serotonin receptors (diagonal elements) compared with the spatial correspondence between different serotonin receptors (off diagonal elements). FC: functional connectivity.
