Extended Data Fig. 5: Spectral power during different vigilance states following bumetanide or VU infusion.
From: Intracellular chloride regulation mediates local sleep pressure in the cortex
a-b. [Cl−]i was manipulated at different time points by locally infusing blockers of NKCC1 (bumetanide) or KCC2 (VU) into S1. Spectral power from REM (‘a’) and wake (‘b’) LFP following infusion of vehicle or bumetanide during the early light period (left), vehicle or bumetanide during the late light period (middle), and vehicle or VU during the late light period (right). No differences were observed in theta frequency (5–10 Hz) during REM sleep following early bumetanide infusion (left; p = 0.615, paired t-test; t = 0.54; df = 5; d = 0.22; black and blue: 6 trials, 5 animals), late bumetanide infusion (middle; p = 0.234, paired t-test; t = 1.35; df = 5; d = 0.55; black and blue: 6 trials, 5 animals), or late VU infusion (right; p = 0.356, paired t-test; t = 1.02; df = 5; d = 0.41; black and red: 6 trials, 4 animals). Data represent mean ± sem. All tests are two sided.
