Extended Data Fig. 6: Local NREM SWA and vigilance state distribution following bumetanide or VU infusion.
From: Intracellular chloride regulation mediates local sleep pressure in the cortex
[Cl−]i was manipulated at different time points by locally infusing blockers of NKCC1 (bumetanide) or KCC2 (VU) into S1. a. Population data showing NREM SWA derived from the S1 LFP (that is local) following infusion in mice that received vehicle or bumetanide during the early light period (left; black and blue: 6 trials, 5 animals), vehicle or bumetanide during the late light period (middle; black and blue: 6 trials, 5 animals), and vehicle or VU during the late light period (right; black and red: 6 trials, 4 animals). b-d. Population data on vigilance states (that is global state) showing the proportion of time spent in NREM sleep (left), REM sleep (middle), and wake (right) following local infusion of vehicle or bumetanide during the early light period (b, black and blue: 5 trials, 5 animals), vehicle or bumetanide during the late light period (c, black and blue: 5 trials, 5 animals), and vehicle or VU during the late light period (d, black and red: 5 trials, 4 animals). Data are plotted in 1-hour intervals. Data represent mean ± sem.
