Extended Data Fig. 1: ITPlxnD1 and PTFezf2 activation patterns during wakeful resting state across mice.
From: Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks
a Variance maps for each mouse (in columns) during quiescent and active episodes averaged over two sessions. b Distribution of activity variance during quiescent (Q) versus active (A) episodes in ITPlxnD1 (blue) and PTFezf2 (green) (n = 12 sessions from 6 mice). c Difference between ITPlxnD1 and PTFezf2 average variance maps for quiescent and active episodes (n = 12 sessions from 6 mice). Only significantly different pixels are displayed (two-sided Wilcoxon rank sum test with p-value adjusted by False Discovery Rate (FDR) = 0.05). Blue pixels indicate values significantly larger in ITPlxnD1 compared to PTFezf2 and vice versa for green pixels. d Distribution of Pearson’s correlation coefficients between quiescent variance maps within ITPlxnD1 (blue), PTFezf2 (green) and between ITPlxnD1 & PTFezf2 (blue green) (66 pairs within ITPlxnD1 & PTFezf2 and 144 pairs between ITPlxnD1 & PTFezf2 in 12 sessions from 6 mice for each cell type). e Distribution of Pearson’s correlation coefficients between active variance maps within ITPlxnD1 (blue), PTFezf2 (green) and between ITPlxnD1 and PTFezf2 (blue green) (66 pairs within ITPlxnD1 & PTFezf2 and 144 pairs between ITPlxnD1 & PTFezf2 in 12 sessions from 6 mice for each cell type). f Distribution of ITPlxnD1 (blue) and PTFezf2 (green) active variance maps projected to the subspace spanned by the top two principal components. g Distribution of ITPlxnD1 (blue) and PTFezf2 (green) quiescent variance maps projected to the subspace spanned by the top two principal components. h Average maps of the 75th (top) and 95th (bottom) percentile df/f value during active and quiescent episodes for ITPlxnD1 and PTFezf2 (n = 12 sessions from 6 mice). *p < 0.05, **p < 0.005, ***p < 0.0005. For box plots, central mark indicates median, bottom and top edges indicate 25th and 75th percentiles and the whiskers extend to extreme points excluding outliers (1.5 times more or less than the interquartile range). All statistics in Supplementary table 1.
