Extended Data Fig. 3: Calcium dynamics of IT^PlxnD1 and PT^Fezf2 at cellular resolution reflect widefield responses.

a Schematic of the whisker stimulation paradigm and the cortical location for two photon imaging (blue circle). b Left: Example field of view (FOV) of IT^PlxnD1 cell bodies and apical dendrites of PT^Fezf2 in the whisker barrel cortex. Right: Example traces from single IT^PlxnD1 cell bodies and apical dendrites of PT^Fezf2. Numbers indicate the corresponding location on the FOV. Magenta bars indicate whisker stimulation events. c Heat map of average single neuron responses of IT^PlxnD1 and PT^Fezf2 classified into 3 groups based on their activity during whisker stimulation from the example FOV. d Average responses across all IT^PlxnD1 and PT^Fezf2 neurons within each group from the example FOV (shaded region indicates ±2 s.e.m). Magenta bars indicate duration of whisker stimulation. e Average responses across all IT^PlxnD1 and PT^Fezf2 neurons from the example FOV (shaded region indicates ±2 s.e.m). f Heat map of average single neuron responses of IT^PlxnD1 and PT^Fezf2 classified based on their activity during whisker stimulation across all mice and sessions (IT^PlxnD1 42 FOV’s from n = 4 mice and PT^Fezf2 36 FOV’s from n = 3 mice). g Average responses across all IT^PlxnD1 and PT^Fezf2 neurons within each group across all mice and sessions (IT^PlxnD1 42 FOV’s from n = 4 mice and PT^Fezf2 36 FOV’s from n = 3 mice, shaded region indicates ±2 s.e.m). h Average responses across all IT^PlxnD1 and PT^Fezf2 neurons from all mice and sessions combined (IT^PlxnD1 42 FOV’s from n = 4 mice and PT^Fezf2 36 FOV’s from n = 3 mice, shaded region indicates ±2 s.e.m). i Proportion of neurons in each group from IT^PlxnD1 and PT^Fezf2.