Extended Data Fig. 3: Classification and spatial distribution of major cell types.
a, Dot plot showing the expression level of top five representative gene markers of each major cell type. b, Matrix plot showing the averaged expression level of canonical gene markers for each glial cell type of the 2,766-genes dataset. Values are normalized for each gene. c, Barplot showing the number of cells per cluster in Control and TauPS2APP mice. Data are represented as mean with 95% CI. Asterisks denote significant differences between Control and TauPS2APP (n = 4 samples per condition, two-sided *P < 0.05, t-test for two independent samples). d, Spatial atlas of top-level cell types in cortex and hippocampus regions of eight samples in the 2,766-gene dataset. Scale bars, 100 µm. e, Representative spatial distribution of cell-type compositions around Aβ plaque for TauPS2APP eight-month samples in both cortex and hippocampus. Stacked bar plot showing the density of each major cell type at different distance intervals to the Aβ plaque. The cell density of each major cell type in each area was included as the reference for comparison (overall). Asterisks denote significantly enriched cell types in each distance interval. One-sided one-sample t-Test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. overall cell density. f, Cell-type composition around Aβ plaque at different distance intervals in both eight- and 13-month samples of the 2,766-gene dataset. Stacked bar plot showing the averaged density of major cell types in the cortex and hippocampus from different distance intervals around the Aβ plaque. The overall cell density of each cell type in each region was included as the reference for comparison (overall). Asterisks denote significantly enriched cell types in each distance interval. One-sided one-sample t-test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. overall cell density.
