Extended Data Fig. 8: Puff-responsive-ANs do not encode backward walking and respond similarly to puffs of air, or CO2.
From: Ascending neurons convey behavioral state to integrative sensory and action selection brain regions
(a-d) Puff-responsive-ANs (SS36112) activity (green) and corresponding spherical treadmill rotational velocities (red, blue, and purple) during (a) long, 2 s CO2-puff stimulation (black) and associated backward walking (orange), (b) short, 0.5 s CO2-puff stimulation, (c) periods with backward walking, and (d) the same backward walking events as in c but only during periods without coincident puff stimulation. Shown are the mean (solid and dashed lines) and 95% confidence interval (shaded areas) of multiple ΔF/F and ball rotation time-series. (e-m) Activity of puff-responsive-ANs (SS36112) from three flies (e-g, h-j, and k-m, respectively) in response to puffs of air (red), or CO2 (black). (e-f, h-i, k-l) Shown are mean (solid and dashed lines) and 95% confidence interval (shaded areas) ΔF/F for ROIs (e,h,k) 0 and (f,i,l) 1. (g,j,m) Mean fluorescence (circles) of traces for ROIs 0 (left) or 1 (right) from 0.7 s after puff onset until the end of stimulation. Overlaid are box plots representing the median, interquartile range (IQR), and 1.5 IQR. Outliers beyond 1.5 IQR are indicated (opaque circles). N = (g) 54 for CO2 and 43 for air (j) 48 for CO2 and 45 for air, and (m) 58 for CO2 and 37 for air-puff epochs. A two-sided Mann-Whitney test (*** p<0.001, ** p<0.01, * p<0.05) was used to compare responses to puffs of CO2 (red), or air (black).
