Extended Data Fig. 9: Lipid-accumulated reactive astrocytes promoted both electrographic and behavioral seizures in epileptic mice.
From: Lipid-accumulated reactive astrocytes promote disease progression in epilepsy
a, Diagram showing the timeline of epileptic model establishment, electrode implantation, EEG recording and MEA recording in APOE−/− mice. b-f, Effect of APOE knockout on KA-induced epilepsy symptoms in mice. b, Behavioral seizures were evaluated at the acute stage using the Racine scale. c, Electrographic seizure-like activities were assessed at the chronic stage by ex vivo MEA recording (WT KA, n = 13; APOE−/− KA, n = 13). The frequency and duration of seizure-like activities were recorded within a 2-min window. d-f, Electrographic seizure-like activities were also assessed by in vivo EEG (WT KA, n = 7; APOE−/− KA, n = 8; APOE−/− KA-CDP choline, n = 10). The number and duration of seizures (EEG) were recorded within a 24-hour window for three continuous days. For b-c and f, data represent the mean ± SEM, n values represent individual animals. Statistical analysis was performed using two-sided unpaired Student’s t-tests (b (left and middle) and c), two-sided Fisher’s exact test (b (right)) and two-way ANOVA with Bonferroni’s post hoc test (f).
