Extended Data Fig. 2: Expression of KCC2 in the varying brain regions in MRS induced by the diverse anesthetics.
(a) Immunoblots of KCC2 expression in eight different brain regions including the cortex, thalamus, hypothalamus, septum, hippocampus, striatum, brainstem, and cerebellum in MRS induced by Pro, Pen, Iso, and Ket (left to right). n = 3 mice in each group of Pro, Ket, Iso groups and their corresponding NA; n = 4 mice in group of Pen and its corresponding NA. (b) Analysis and comparison of changes of KCC2 expression in the different brain regions in MRS induced by the diverse anesthetics (data from the corresponding groups in a). n = 3 mice in each group of Pro, Ket, and Iso and 4 mice in Pen. Unpaired Student’s t-test (two-tailed). The P values (Pro, Pen, Ket, Iso) in group of cortex: 0.7901, 0.3085, 0.0358, and 0.021; thalamus: 0.0072, 0.0028, 0.0424, and 0.0451; hypothalamus: 0.0005, 0.0016, 0.0028, and 0.0042; septum: 0.2957, 0.0114, 0.8098, and 0.4869; hippocampus: 0.8521, 0.046, 0.0338, and 0.0482; striatum: 0.0945, 0.02641, 0.0333, and 0.7185; brainstem: 0.0138, 0.1061, 0.0024, and 0.2396; cerebellum: 0.0245, 0.1101, 0.9358, and 0.9971. Mean ± s.e.m. MRS versus NA in the same anesthetic group, *P < 0.05, **P < 0.01, ***P < 0.001. Detailed statistics information is provided in Supplementary Table 1.
