Extended Data Fig. 3: Immunofluorescence mapping of expression of KCC2 in the varying brain regions in MRS.

(a–e) Immunofluorescence of KCC2 expression in the thalamus, hypothalamus, cortex, and hippocampus (a) and in the seven sub-nuclei of the thalamus and hypothalamus including MHb, LHb, paraventricular thalamic nucleus (PV)(b), and the VPM (c), and sub-nuclei of the hypothalamus including lateral hypothalamic area (LH), substantia innominate (SI)(d), and paraventricular hypothalamic nucleus (PVN)(e). (f) Data analysis and summary. Mean ± s.e.m., n = 4 mice in each group. One-way ANOVA with Dunnett’s multiple comparisons. P > 0.9999 (NA versus Pro, cortex), P = 0.309 (NA versus Pen, cortex), P = 0.9946 (NA versus Pro, hippocampus). NS = not significant. ****P < 0.0001 in all the others comparisons as indicated. Detailed statistics information is provided in Supplementary Table 1.