Extended Data Fig. 5: Effects of knockdown of KCC2 in the sub-nuclei of the thalamus and hypothalamus on the effect of propofol and isoflurane anesthesia. | Nature Neuroscience

Extended Data Fig. 5: Effects of knockdown of KCC2 in the sub-nuclei of the thalamus and hypothalamus on the effect of propofol and isoflurane anesthesia.

From: Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus

Extended Data Fig. 5

(a–f) Representative images showing AAV2/9-hSyn-mCherry-miRNAi (KCC2) transfected LHb, MHb, and PV of the thalamus and LH, PVN, and SI of the hypothalamic nuclei (left two columns). AAV2/9- hSyn-mCherry-miRNAi (KCC2)-induced KCC2 knockdown in these nuclei did not affect propofol (middle two columns) and isoflurane (rightmost column) anesthesia. Number of mice in each of the groups (from a to f): propofol, n = 5, 6, 5, 6, 6, and 6; isoflurane, n = 5, 6, 4, 6, 6, and 6). Unpaired Student’s t-test (two-tailed). The P values (Pro, Iso) in each group: a, P = 0.6454, 0.963; b, P = 0.733, 0.9746; c, P > 0.9999, = 0.9538; d, P = 0.7423, 0.9286; e, P = 0.7294, 0.1372; and f, P = 0.9365. Mean ± s.e.m. NS = not significant. Detailed statistics information is provided in Supplementary Table 1.

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