Extended Data Fig. 7: BDNF expression in thalamus and effects of Fblx4 overexpression in the VPM on KCC2 expression in mice with or without propofol anesthesia. | Nature Neuroscience

Extended Data Fig. 7: BDNF expression in thalamus and effects of Fblx4 overexpression in the VPM on KCC2 expression in mice with or without propofol anesthesia.

From: Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus

Extended Data Fig. 7

(a) Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group. Unpaired Student’s t-test (two-tailed): P = 0.372. (b) Representative western blot images showing time course of changes of expression of KCC2 in the VPM before, during, and recovery from propofol anesthesia following overexpression of Fblx4 by injection of LENTAI–EGFP(Fblx4). (c) Data analysis and summary (from b). n = 3 mice in each group at each checking point. One-way ANOVA with Dunnett’s multiple comparisons and the P values (the specific data point versus NA-uninfected): > 0.9999 (5), < 0.0001 (15), < 0.0001 (30), 0.4151 (90), 0.9998 (120). The P values (the specific data point versus NA-infected): 0.0013 (5), < 0.0001 (15), < 0.0001 (30), 0.0045 (90), 0.9997 (120). Unpaired Student’s t-test (two-tailed). The P values (NA-infected versus NA-uninfected): 0.0041. The P values (infected versus uninfected at the specific data point): 0.0018 (5), 0.0357 (15), 0.3992 (30), 0.0144 (90), and 0.0334 (120). Mean ± s.e.m. ****P < 0.0001, +P < 0.05, ++P < 0.01, ##P < 0.01, ####P < 0.0001. Detailed statistics information is provided in Supplementary Table 1.

Source data

Back to article page