Extended Data Fig. 8: Alterations of expression of phosphorylation of KCC2 Thr1007 in MRS induced by the diverse anesthetics. | Nature Neuroscience

Extended Data Fig. 8: Alterations of expression of phosphorylation of KCC2 Thr1007 in MRS induced by the diverse anesthetics.

From: Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus

Extended Data Fig. 8

(a–c) Immunoblots showing up-regulation of KCC2 Thr1007 phosphorylation in the whole brain (a), thalamus (b), and hypothalamus (c) in MRS with Pro, Pen, Ket, or Iso anesthesia. Phosphorylation of KCC2-Thr1007 increased in the regions where expression of KCC2 decreased (see Extended Data Fig. 2). n = 3 mice in each group of Pro, Ket, Iso and the corresponding NA, n = 4 mice in group of Pen and its corresponding NA. Unpaired Student’s t-test (two-tailed) and the P values (Pro, Pen, Ket, and Iso): a, 0.0146, 0.0377, 0.0293, and 0.0019; b, 0.0006, 0.0122, 0.0401, and 0.012; c, 0.0107, 0.0013, 0.0005, and 0.0437. (d) Immunoblots showing down-regulation of KCC2 Thr1007 phosphorylation in the septum, striatum, cortex, and brainstem. Phosphorylation of KCC2-Thr1007 decreased in the regions where expression of KCC2 increased (see Extended Data Fig. 2). n = 3 mice in each group of Pro, Ket, Iso and the corresponding NA, n = 4 mice in group of Pen and its corresponding NA. Unpaired Student’s t-test (two-tailed) and the P values (Pro, Pen, Ket, and Iso): 0.0154, 0.0088, 0.0003, 0.0322, and 0.002. Mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. Detailed statistics information is provided in Supplementary Table 1.

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