Extended Data Fig. 9: Overexpression of mutant KCC2-Thr1007 (Thr1007A) in the VPM by loci injection of LENTAI–hSyn-EGFP-Thr1007A. | Nature Neuroscience

Extended Data Fig. 9: Overexpression of mutant KCC2-Thr1007 (Thr1007A) in the VPM by loci injection of LENTAI–hSyn-EGFP-Thr1007A.

From: Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus

Extended Data Fig. 9

(a) Coronal sections from anterior to posterior confirmed the site of virus injection and infection were well confined to the VPM region. (b) Immunoblots confirmed that overexpression of Thr1007A significantly prevented phosphorylation of KCC2-Thr1007 and degradation of KCC2 in the VPM in MRS with Pen, Ket, or Iso. Left, representative western blots. Right, data analysis and summary. n = 3 mice in each group. One-way ANOVA with Dunnett’s multiple comparisons. The P values (Thr1007/GAPDH): 0.0018 (Pen-Thr1007A versus Pen-NC), 0.0005 (Ket-Thr1007A versus Ket-NC), 0.0001 (Iso-Thr1007A versus Iso-NC). The P values (KCC2/GAPDH): < 0.0001 (Pen-T1007A versus Pen-NC), < 0.0001 (Ket-Thr1007A versus Ket-NC), 0.0055 (Iso-Thr1007A versus Iso-NC). Mean ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.00001. Detailed statistics information is provided in Supplementary Table 1.

Source data

Back to article page