Extended Data Fig. 8: Comparison of spatial placement of prelimbic neurons projecting to diverse brain areas via retrograde labelling from projection areas.
From: Prefrontal engrams of long-term fear memory perpetuate pain perception
In panels a-e, the left panels show schematics of injections of YFP- or mCherry-expressing retro-AAV virions either in the indicated projection targets of prelimbic cortex (PL). Middle panels show the injection areas and the two panels on the right show example images from confocal microscopy revealing the juxtaposition or intermingling of PL neurons that project to these discrete and distant areas. The viral expression was bilaterally verified in n = 2 mice per group. Abbreviations: MD: mediodorsal thalamus; PAG: periaqueductal gray; AI: anterior insula; BLA: basolateral amygdala; ACC: rostral anterior cingulate cortex; PI: posterior insula. Scare bars represent 200 µm in the middle panels and 50 µm in panels on the right.
