Extended Data Fig. 3: Additional data on interneuron spatial selectivity and generalized-linear model of interneuron activity during spatial navigation.
From: Inhibitory control of sharp-wave ripple duration during learning in hippocampal recurrent networks
(a) Spatial information for significantly tuned interneurons, broken down by both region and subtype. Immobility-active CB/SATB1- neurons were silenced during locomotion and were thus not considered in this analysis. Data from 188 cells in n = 9 mice. Significance values over individual violin plots show the results of (one-way) signed-rank tests. (b) Within-day spatial stability of all interneurons, broken down by both region and subtype. Plotted as in A. Significance values over individual violin plots show the results of (one-way) signed-rank tests. Significance values over pairs of violin plots show results from (two-way) ranked-sum tests (only significant differences are shown). Data from 152 cells in n = 9 mice. (c) Across-day spatial stability of all interneurons, broken down by both region and subtype. Plotted as in A. Significance values over individual violin plots show the results of (one-way) signed-rank tests. Significance values over pairs of violin plots show results from (two-way) ranked-sum tests (only significant differences are shown). Data from 142 cells in n = 9 mice. (d) Left: Summary of the fraction of positively tuned CA2 interneurons, broken down by subtype (PVBC: 0.343 ± 0.341, AAC: 0.455 ± 0.267, SOM: 0.405 ± 0.339, CCK: 0.685 ± 0.228, CB/SATB1 + : 0.0 ± 0.0). CA2 CCK cells were more likely to be positively spatially tuned cells than CA2 CB/SATB1+ cells (one-way ANOVA with post-hoc multiple testing correction, p = 0.048). Immobility-active CB/SATB1- neurons were silenced during locomotion and were thus not considered in this analysis. Each data point represents an imaging session. PVBC data from 20 imaging sessions, AAC data from 22 sessions, SOM data from 14 sessions, CCK data from 9 sessions, and CB/SATB1 + data from 2 sessions; data from n = 9 mice. Data reported as mean ± s.d. Right: Same data as on the left, but for negatively tuned CA2 interneurons (PVBC: 0.304 ± 0.261, AAC: 0.270 ± 0.293, SOM: 0.690 ± 0.226, CCK: 0.685 ± 0.228, CB/SATB1 + : 0.75 ± 0.25). Significant differences in the fraction of negatively tuned CA2 interneurons by subtype are indicated (one-way ANOVA with post-hoc multiple testing correction: p(CCK-PVBC) = 0.0075, p(CCK-AAC) = 0.0024, p(SOM-PVBC) = 0.0012, p(SOM-AAC) = 0.001). Immobility-active CB/SATB1- neurons were silenced during locomotion and were thus not considered in this analysis. Each data point represents an imaging session. PVBC data from 20 imaging sessions, AAC data from 22 sessions, SOM data from 14 sessions, CCK data from 9 sessions, and CB/SATB1 + data from 2 sessions; data from n = 9 mice. Data reported as mean ± s.d. (e) Example of 100 seconds of real interneuron activity during locomotion and the predicted activity from a GLM. The predicted activity for each cell was calculated based on 4 predictor behavioral variables: velocity, position, licking, and water delivery (see Methods). (f) Comparison of the GLM weights for each cell for the velocity predictor, separated by both subtype and region. Only cells for which the velocity predictor in the model was a significant predictor are shown. Significance values over individual violin plots show the results of (one-way) signed-rank tests. Significance values over pairs of violin plots show results from (two-way) ranked-sum tests. Only significant differences are shown. Data from 170 cells from n = 9 mice. (g) Same data as shown in F, but now for the licking predictor. Only cells for which the licking predictor in the model was significant are shown. No significance at the subtype or region level was found. Data from 20 cells in n = 9 mice. (h) Same data as shown in F, but now for the reward predictor. Only cells for which the reward predictor in the model was significant are shown. No significance at the subtype or region level was found. Data from 56 cells from n = 9 mice. (i) Same data as shown in F, now for the position predictor. Only cells for which the position predictor in the model was significant are shown. Significance values over pairs of violin plots show results from (two-way) ranked-sum tests. Only significant differences are shown. Data from 45 cells from n = 9 mice.
