Extended Data Fig. 4: Expression pattern of ATB-split-GAL4 lines.

E a, Representative images of the posterior abdomen from two of seven ATB-2>mCD8-GFP females, lateral (left) and ventral aspects (right). mCD8-GFP expression, membrane of ATB neurons (green); autofluorescence, abdominal cuticle (magenta). Background, overlaid bright-field images reveal extended bristles. Here and in b-d, scale bar, 50 μm. b, Representative image of the ventral nerve cord (left) and abdominal neuromere (right) from two ATB-2>mCD8-GFP females, stained with anti-GFP (membrane of ATB neurons, green) and nc82 (synaptic neuropil, magenta). Black bars flanking left image, region shown in higher resolution at right. c, Representative images of the brain from one of two ATB-1>mCD8-GFP females (left) and from one of two ATB-2>mCD8-GFP females (right), stained with anti-GFP (membrane of ATB neurons, green) and nc82 (synaptic neuropil, magenta). d, Representative images of the brain (left, one of six flies) and ventral nerve cord (right, one of six flies) from ATB-1>Kir2.1-T2A-tdTomato females, stained with anti-DsRed (ATB neurons co-expressing tdTomato and Kir2.1, green) and nc82 (synaptic neuropil, magenta). Foreleg expression is sparse in these flies (0.67 + /− 0.65 efferents per side in the dorsal prothoracic nerve; n = 12 nerves from six flies; mean ± s.d.).