Extended Data Fig. 6: Experimental validation of adDEGs.
From: Single-nucleus multiregion transcriptomic analysis of brain vasculature in Alzheimer’s disease
a. Chromogenic RNAscope for ABCC9 and SLC6A1. Prefrontal cortex brain sections (n = 4 for AD and n = 4 for nonAD) were sectioned at 20 µm (3 images per slide), then stored at -80 °C. Sections were then prepared for RNAscope using the manufacturer’s instructions. Scale bar, 20 µm. b. Quantification of SLC6A and ABCC9 double-positive cells per image. P value was calculated by t-test. Data are presented as mean values +/− standard deviation. c. Collagen-4 and lectin-488 immunohistochemistry. Scale bar, 50 µm. d. Additional images of collagen-4 immunohistochemistry. Scale bar, 20 µm. e. Quantification of collagen-4 signal intensity. P value was calculated by t-test (n = 4 for AD and n = 4 for nonAD individuals). Data are presented as mean values +/− standard deviation.
