Extended Data Fig. 2: Proteinase K efficiently exposes the C-terminal amyloid core of TDP-43 filaments.
From: Seeding the aggregation of TDP-43 requires post-fibrillization proteolytic cleavage
a and b) EM images of TDP-43 filaments immunogold labeled with (a) N-terminal (N-ter-AB) and (b) C-terminal (C-ter-AB) specific TDP-43 antibodies. c) Dot blot of FL TDP-43 filaments stained with full-length (FL-AB), N-ter-AB, and C-ter-AB specific TDP-43 antibodies. d) Dot blot analysis of trypsin (T) and proteinase K (PK) treated TDP-43 filaments at different time points stained using N-terminal specific TDP-43 antibody. Bar graph shows the quantified antibodies staining intensities of the respective dot blot images. Error bars represent SD, n = 4 independent experiments. e) Representative electron micrographs of trypsin (top row) and PK (bottom row) treated TDP-43 filaments at different time points.
