Extended Data Fig. 6: Sparse lentiviral expression of TeNT in striatal SPNs does not disrupt the basal functional properties of afferent synapses formed onto SPNs.
From: Postsynaptic synucleins mediate endocannabinoid signaling
a, Representative image showing lentiviral expression of GFP-2A-TeNT in the striatum (1 of 8 slices). b, High magnification images. Top: DAPI, middle: GFP, bottom: overlay. c, Quantification of infection efficiency (GFP + / DAPI+): 10.28 ± 1.10% cells, n = 8 slices / 4 mice. d, Representative traces of evoked corticostriatal EPSCs in WT (GFP-) and TeNT-infected (GFP+) SPNs across a range of stimulation intensities. e, Input-output curves (GFP-: n = 12 cells / 5 mice; GFP+: n = 14 cells / 6 mice; p = 0.787). f, Representative traces of mEPSC recordings from WT (GFP-) and TeNT-expressing (GFP+) cells. g-j, Summary of mEPSC frequency (GFP-: n = 17 cells / 5 mice, 2.81 ± 0.22 Hz; GFP+: n = 18 cells / 5 mice, 2.64 ± 0.20 Hz; p = 0.680). h, Cumulative distribution of mEPSC inter-event intervals. i, Summary of mEPSC amplitude (GFP-: 16.78 ± 0.62 pA; GFP+: 16.65 ± 0.55 pA; p = 0.987). j, Cumulative distribution of mEPSC amplitudes. Data are mean ± SEM. (c, g, i) Box plots are depicted as mean (center), first/third quartile (lower/upper box limits), and minima/maxima (bottom/top whiskers). Statistical significance was assessed by two-sided tests, including 2-way repeated measures ANOVA (e) and Mann-Whitney tests (g, i) (n.s. non-significant).
