Extended Data Fig. 7: 2-photon Ca2+ imaging in SPN dendrites shows that lentiviral expression of TeNT does not alter SPN dendritic Ca2+ signaling.
From: Postsynaptic synucleins mediate endocannabinoid signaling
a, Representative 2-photon image of a SPN (1 of 8 cells). b, Images of dendritic segments in red (Alexa594, 50 µM) and green (Fluo-5F, 300 µM) channels while cells were depolarized from -70 mV to 0 mV from WT (top) and TeNT-expressing (bottom) SPNs. c, Summary of depolarization-induced changes in dendritic Ca2+ (GFP-, n = 33 dendrites / 4 cells / 3 mice, -70 mV: 0.25 ± 0.11, 0 mV: 1.45 ± 0.53; p = 3.77e-9; GFP + / TeNT-expressing SPNs, n = 36 dendrites / 4 cells / 3 mice, -70 mV: 0.23 ± 0.14, 0 mV: 1.42 ± 0.30; p = 3.77e-9; GFP- vs. GFP+, p = 0.84). Data are mean ± SEM. (c) Box plots are depicted as mean (center), first/third quartile (lower/upper box limits), and minima/maxima (bottom/top whiskers). Statistical significance was assessed by two-sided ANOVA with multiple comparisons (c) (**** p < 0.0001; n.s. non-significant).
