Fig. 5: α-Syn membrane interaction domain is required for eCB-LTD.
From: Postsynaptic synucleins mediate endocannabinoid signaling
a–f, eCB-LTD is absent in GFP− SPNs (a), rescued by full-length human α-Syn (b) but not rescued by the expression of mutant A11P/V70P α-Syn (c) or by α-Syn harboring the PD mutation A30P (d) (GFP−, pooled: n = 16 cells, 7 mice, 93.84 ± 3.06%; GFP+, hSNCA: n = 7 cells, 3 mice, 59.08 ± 4.90%, P = 4.98 × 10−7; A11P/V70P: n = 11 cells, 6 mice, 95.40 ± 3.17%; P = 0.986; A30P: n = 10 cells, 7 mice, 95.75 ± 3.59%; P = 0.977). e, Summary of EPSC amplitudes. f, Summary of PPRs for Syn-tKO cells infected with α-Syn (GFP+, hSNCA baseline: 1.14 ± 0.09; post-DHPG: 1.35 ± 0.11; P = 0.016; GFP−, pooled baseline: 0.89 ± 0.02; post-DHPG: 0.92 ± 0.03; P = 0.255; A11P/V70P baseline: 1.07 ± 0.09; post-DHPG: 1.05 ± 0.08; P = 0.496; A30P baseline: 1.12 ± 0.11; post-DHPG: 1.10 ± 0.07; P = 0.695). Data are mean ± s.e.m. e,f, Box plots are depicted as mean (center), first/third quartiles (lower/upper box limits) and minima/maxima (bottom/top whiskers). Statistical significance was assessed by two-sided tests, including ANOVA with multiple comparisons (e) and Wilcoxon signed test (f) (****P < 0.0001; *P < 0.05; NS, not significant).
