Extended Data Fig. 1: The triple α/β/γ-synuclein KO has no effect on basal corticostriatal synaptic transmission, but blocks eCB-LTD in aged Syn-tKO mice.
From: Postsynaptic synucleins mediate endocannabinoid signaling
a, Representative traces of evoked corticostriatal EPSCs in WT and Syn-tKO SPNs from aged mice (16–18 months old) across a range of stimulation intensities. b, Input-output curves in WT and Syn-tKO mice (WT: n = 14 cells / 6 mice; Syn-tKO: n = 12 cells / 5 mice; p = 0.960). c-f, DHPG-mediated eCB-LTD in aged (16–18 months old) WT mice is fully blocked by the CB1R antagonist AM251 (10 µM) (WT: n = 9 cells / 4 mice, 73.88 ± 2.74%; WT + AM251: n = 7 cells / 4 mice, 97.06 ± 3.20%; p = 3.026e-5); top, representative traces. d, eCB-LTD is abolished in aged Syn-tKO mice (Syn-tKO: n = 9 cells / 6 mice, 92.57 ± 2.57%; p = 1.955e-4); top, representative trace. e, Summary of EPSC amplitudes. f, Summary of PPRs (WT baseline: 1.02 ± 0.04; post-DHPG: 1.15 ± 0.06; p = 3.9e-3; Syn-tKO baseline: 0.95 ± 0.03; post-DHPG: 0.99 ± 0.03; p = 0.301). Data are mean ± SEM. (e, f) Box plots are depicted as mean (center), first/third quartile (lower/upper box limits), and minima/maxima (bottom/top whiskers). Statistical significance was assessed by two-sided tests, including 2-way repeated measures ANOVA (b), ANOVA with multiple comparisons (e), and Wilcoxon signed tests (f) (**** p < 0.0001; *** p < 0.001; ** p < 0.01; n.s. non-significant).
