Fig. 2: Disruption of orexin cell function leads to observable pupil size differences.
From: Control and coding of pupil size by hypothalamic orexin neurons
a, The pupil was recorded during 20-Hz optostimulation of LH orexin neurons in ALM- or vehicle-injected, isoflurane anesthetized mice. b, An example pupil size trace from one mouse during orexin cell optostimulation after ALM or vehicle injection. The green shaded area indicates optostimulation. c, Absolute pupil size across mice (mean ± s.e.m. of n = 5 mice). d, Left, within mouse comparison of baseline pupil diameters after vehicle or ALM injections (n = 5 mice, one-tailed paired t-test, t = 2.8, P = 0.02). Right, within mouse comparison of change in pupil diameter from baseline to peak during laser stimulation (one-tailed paired t-test, t = 4.75, P = 0.0045). e, Pupil diameter of orx-DTR+ and orx-DTR−; DT-injected mice were recorded during head-fixed running on a wheel. f, Mean speed within k-means-identified clusters of equivalent running bouts from DTR+ and DTR− mice (mean ± s.e.m. of n = 7 orx-DTR+ and n = 8 orx-DTR− mice). g, Pupil dynamics (means ± s.e.m.) corresponding to the running bouts shown in f. h, Left, comparison of baseline pupil diameters (at −2 s from run onset) for DTR− (n = 8) and DTR+ (n = 7) mice (one-tailed t-test, t = 1.8, P = 0.048). Right, comparison of pupil diameter during the run bout (at +6 s from run onset; one-tailed t-test, t = 0.3, P = 0.39). *P < 0.05; NS, not significant.
