Fig. 1: Hippocampal RyR2 channels are remodeled and leaky in patients with heart failure.
a,b, Representative SDS–PAGE analysis and quantification of modified RyR2 and calstabin2 immunoprecipitated from hippocampi of controls and individuals with HF (IP RyR2; bands normalized to total RyR2). Control (CTRL), n = 4); HF, n = 9. c, Single-channel recordings of RyR2 incorporated in planar lipid bilayers with 150 nM Ca2+ in the cis chamber, corresponding to representative experiments performed using human hippocampal samples from controls and HF patients (two traces from two different controls and individuals with HF are shown) d, Po, To and Tc of RyR2 channels in controls (n = 5) and HF (n = 9) hippocampi. e, ER Ca2+ leak measured in microsomes from control (n = 4) and HF participant (n = 9) hippocampi. f, Bar graphs represent the quantification of microsomal Ca2+ leak as the percentage of uptake in controls (n = 5) and HF individuals (n = 9). Individual values are shown with the mean ± s.e.m. (t-test *P < 0.05, controls versus HF individuals). Data are derived from biologically independent samples. All statistical tests were two sided. a.u., arbitrary units.
