Extended Data Fig. 2: Cognitive function in RyR1-S2844D mice.
a) Open field test using WT mice (n = 10) and a mouse model with leaky RyR1 channels (S2844D) (n = 21). Ratios of total time spent in the center area versus periphery area within first 3 min and second 3 min are shown. b) Elevated plus maze test in WT mice (n = 10) and S2808D (n = 21). Ratios of time spent in the open-arm versus closed-arm are shown. c) Novel object recognition test in WT mice (n = 10) and S2808D (n = 21). Discrimination index is shown. d) Morris water maze test (learning curves) in WT mice (n = 10) and S2808D (n = 21). e) Probe trials after escape platform removed in the same groups showing the total duration spent in the target quadrant in WT mice (n = 10) and S2808D (n = 21). f) Number of target crossings in WT mice (n = 10) and S2808D (n = 21). g) Heat maps showing the latency from each group at Day 2 and Day 5. Individual values are shown with mean ± SEM. T-test was used in panel A-C, E-F, * p < 0.05 in panel A shows significance between the first 3 min and second 3 min of each group). Two-way ANOVA was used in panel D. Tukey’s test post-hoc correction for multiple comparisons was used. All statistical tests were two-sided. Data are derived from biologically independent samples.
