Fig. 5: Adrenergic agonist and RyR2 Ser2808 phospho-mimetic mutation deplete endoplasmic reticulum Ca2+ stores in primary hippocampal neurons. | Nature Neuroscience

Fig. 5: Adrenergic agonist and RyR2 Ser2808 phospho-mimetic mutation deplete endoplasmic reticulum Ca2+ stores in primary hippocampal neurons.

From: Heart failure-induced cognitive dysfunction is mediated by intracellular Ca2+ leak through ryanodine receptor type 2

Fig. 5

a, Representative images of 14-d cultured hippocampal neurons stimulated with 10 mM caffeine. For each condition, the Ca2+ levels are shown at baseline, during stimulation and at recovery. b, Quantification of caffeine-induced Ca2+release (F/F0) in response to 10 mM caffeine in neurons from wild-type (WT; n = 50), WT + S107 (n = 30), WT + isoproterenol (ISO; n = 26), WT + ISO + propranolol (n = 35), neurons expressing RyR2-p.Ser2808Asp untreated (S2808D; n = 70) and treated with S107 (p.Ser2808Asp + S107; n = 54). Individual values are shown with the mean ± s.e.m. (t-test *P < 0.05). Scale bar, 10 μm. A reduction in caffeine-induced Ca2+ release indicates a Ca2+-depleted ER due to persistent RyR2-mediated Ca2+ leak. Data are derived from biologically independent samples. All statistical tests were two sided.

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