Fig. 7: Altered synaptic protein expression in heart failure.
a, Cohort plot representation of differentially expressed synaptic proteins (SHAM versus MI) from six significantly enriched synaptic transmission GO terms and generated by GOplot. The color map represents fold change of proteins (log2 scale). Selected proteins in the SNARE pathway are highlighted in red (upregulated) or green (downregulated). b,c, Immunoblots showing total expression of SNAP25, VAMP8, SYT2 and CPLX3, normalized to GAPDH in the hippocampi of controls (n = 4) and individuals with HF (n = 9). Individual values are shown with the mean ± s.e.m. (t-test *P < 0.05, control versus individuals with HF). d,e, Immunoblots showing total expression of SNAP25, VAMP8, SYT2 and CPLX3, normalized to GAPDH in the hippocampi of SHAM (n = 6), MI (n = 6), MI + ARM036 (n = 6), MI + S107 (n = 6), MI + propranolol (n = 4) and MI + SD-208 (n = 4) mice. Individual values are shown with the mean ± s.e.m. One-way ANOVA and Tukey’s test post hoc correction for multiple comparisons, *P < 0.05, SHAM versus MI or MI + ARM036; #P < 0.05 MI versus MI + S107, MI + propranolol or MI + SD-208. Data are derived from biologically independent samples. All statistical tests were two sided.
