Extended Data Fig. 6: Manipulating M1 temperature did not produce discernible effects on timing judgments.
From: Using temperature to analyze the neural basis of a time-based decision
(a) Average discrimination performance in the fixation version of the interval discrimination task at the onset of M1 temperature manipulations. Psychometric functions fit to cross-animal averages (N = 4) of temperature-split psychophysical data, respectively shown as solid lines and markers of matching color (mean ± s.e.m.). Bottom right inset: Average differences in proportion of long choices from each manipulation condition to control (mean ± propagated s.e.m.). Top-left inset: Marginal posterior distributions of the threshold parameter for each condition’s psychometric fit. Solid black lines represent the M.A.P. point estimates implicit in the fits shown in the main axes. (b) Animal-split discrimination behavior. Markers represent M.A.P. estimates and transparent patches the corresponding 95% confidence intervals of threshold parameters fit to individual animals’ performance on control (vertical axis) versus manipulation blocks (horizontal axis). Inset: Distribution of threshold differences between manipulation and control conditions. Markers represent individual animal differences (mean ± s.e.m.). (c) Effect of motor cortical temperature manipulations on psychophysical threshold (all non-significant |ts(3)| = [0.93, 2.90], P = [0.06, 0.42]; repeated measures ANOVA, F(2, 9) = 1.93, P = 0.20). Markers represent individual threshold dilations, linked within animals by thin solid black lines. Boxplots show animal means (horizontal black lines) and s.e.m. (colored bars). (d) Threshold dynamics aligned to and across block transitions. Condition-split cross-animal average thresholds (mean ± s.e.m.) were computed using trials that fell into a sliding window lasting 90 s (half the block duration) that was swept from the preceding to the succeeding control blocks in increments of 9 s. Each marker corresponds to one sweep, and its color shading denotes the fraction of that sweep’s window that was inside a control block (with black markers corresponding to 100% control trials), and by extension its complement that fell in a manipulation block (with pure manipulation colors corresponding to 100% manipulation trials).
