Fig. 4: Sparse, sequential activity is preserved in ChC manipulation.
From: An adaptive behavioral control motif mediated by cortical axo-axonic inhibition
a, Example image of the axonal projection of ChCs (expressing mCherry, in magenta) to AISs (post hoc AnkG staining, in cyan) of neighboring L2/3 neurons (expressing GCaMP6s, in green, n = 9 mice). Axonal boutons of ChCs innervating an AIS (right). Yellow arrowheads indicate putative cartridges associated with AISs. Gray dashed lines indicate laminar boundaries for cortical layers 1 to 2/3. b, Schematic of selective abolition of GABA release from ChCs in M2 by expressing TeTxLC in ChC-Flp mice (Nkx2.1-2a-CreER::Flex-FlpO mice). c, An example of movement speed (top), the normalized activity of neurons (middle) and probability of maximum neuron activation (bottom) aligned to movement onsets in ChC-TeTxLC and ChC control. d, Average movement speed (top) and corresponding changes in the probability of maximum neuron activation (bottom) aligned to movement onsets. e, Example pairwise correlation matrices of ChC-TeTxLC and ChC control mice. f, Population fraction of neuronal pairs with positive and negative correlation (n = 14,082 pairs for ChC-TeTxLC, n = 57,956 pairs for ChC control; two-tailed chi-square test, χ2 = 1.21, P = 0.27). g–j, The activity of ChCs related to the movement initiation. g, The normalized activity of ChCs aligned to movement onset (35 cells from three mice). h, The normalized activity of neurons aligned to movement onset in the ChC-TeTxLC group (n = 982 cells from nine mice). i, Average ΔF/F traces of ChCs (top) and neurons in the ChC-TeTxLC group (bottom) aligned to movement onset. j, Probability distributions of peak activity timing of ChCs and neurons in the ChC-TeTxLC group aligned to movement onset. Error bars and shading indicate s.e.m. NS, not significant.
