Fig. 6: Variability of ChC activity increases during learning.
From: An adaptive behavioral control motif mediated by cortical axo-axonic inhibition
a, Representative field of view showing L2 ChCs expressing GCaMP6 (n = 3 mice). b, Example fluorescence traces from ChCs that show increased activity during episodes of locomotion. c, Movement speed and the heat map of ChC activity during navigation in sessions 1 and 7. The synchronous activation of ChCs in session 1 during locomotion becomes asynchronous in session 7. d, Changes in the correlation between a pair of ChCs (n = 189 pairs in 35 cells from three mice; two-tailed Wilcoxon signed-rank test, Z = 11.92, P = 0). e, Example correlation matrix of ChCs in sessions 1 and 7. f, Various temporal relationships between activity of ChC (magenta) and neighboring M2 neurons (gray) during episodes of locomotion in ChC-hM4Di mice. g, Cell-to-cell Pearson’s correlation of ChC–M2 neuron pairs in sessions 1 and 7 during locomotion and rest epochs (four ChC-hM4Di mice, n = 1,957 pairs for session 1, two-tailed two-sample Kolmogorov–Smirnov test, locomotion versus rest, D = 0.076, P = 2.12 × 10−5; n = 1,723 pairs for session 7, locomotion versus rest, D = 0.28, P = 4.049 × 10−61). ***P < 0.001; shadings indicate s.e.m.
