Extended Data Fig. 3: Distribution of the FNE-IO and FNI-IO neurons in the FN.
From: Excitatory nucleo-olivary pathway shapes cerebellar outputs for motor control
a, Tracing strategy for labeling excitatory mcMAO-projecting FN neurons (termed FNE-IO neurons). AAV9-FLEX-tdTomato was injected into the FN, and AAVretro-Cre was injected into the mcMAO. CTB-Alexa647 was co-injected with AAVretro-Cre to label the injection site, showing colocalization of FN axons (red) and Alexa647 (yellow) in the mcMAO. b, Histological sections, and the corresponding annotation of FNE-IO neurons from a representative animal. c, d, Same as (a, b), but for labeling the inhibitory lcMAO-projecting FN neurons (termed FNI-IO neurons). AAV9-FLEX-tdTomato was injected into the FN, and AAVretro-Cre (mixed with CTB-Alexa647) was injected into the lcMAO. Example image showing colocalization of FN axons (turquoise) and Alexa647 (yellow) in the lcMAO region. e, Distribution of FNE-IO (red) and FNI-IO neurons (turquoise, n = 3 mice for each group, mean ± s.e.m.). Upper: caudal and top views of three-dimensionally rendered FN and the superimposed FNE-IO and FNI-IO neurons. Lower: quantifications of these two populations along the rostro-caudal, latero-medial, and dorso-ventral axes. Stereotaxic abbreviations, D: dorsal, L: lateral, R: rostral.
