Extended Data Fig. 7: Brain-wide quantification of axonal projection from FNE-IO neurons.
From: Excitatory nucleo-olivary pathway shapes cerebellar outputs for motor control
Related to (Fig. 5a-c). a, Example images of different brain regions receiving FNE-IO neuron projection. Anterograde AAV9-FLEX-GFP was injected into the left FN and AAVretro-Cre was injected into the contralateral mcMAO to specifically label the FNE-IO neurons (red asterisk in the first image). See the tracing schematic in (Fig. 5a). b, Fractions of axon collaterals in six major brain regions. Labeling intensity is normalized to the total labeled axons of each mouse. Gray dots: individual mice, black dots and bars: mean ± s.e.m. (n = 3 mice). c, Comparison of the ipsilateral and contralateral projections in six major brain regions (see color codes in b). Gray dots: individual mice, bars: mean + s.e.m. (n = 3 mice). d, Projection density matrix of the top 50 regions targeted by FNE-IO axons. Each row represents one animal. Nuclei are sorted from left to right based on the projecting density. The mcMAO is indicated by a white rectangle. IRN: intermediate reticular nucleus, MDRN: medullary reticular nucleus, MV: medial vestibular nucleus, SPIV: spinal vestibular nucleus, PRP: nucleus prepositus, GRN: gigantocellular reticular nucleus, PAG: periaqueductal gray, MRN: midbrain reticular nucleus, PPN: posterior pretectal nucleus, PRNr: pontine reticular nucleus, TRN: tegmental reticular nucleus, RN: red nucleus, VTA: ventral tegmental area, MD: mediodorsal nucleus of thalamus, VM: ventral medial nucleus of the thalamus, PO: posterior complex of the thalamus, VPM: ventral posteromedial nucleus of the thalamus, VAL: ventral anterior-lateral complex of the thalamus, SMT: submedial nucleus of the thalamus, FF: fields of Forel, ZI: zona incerta.
