Extended Data Fig. 8: Single-cell projection patterns of FN^E-IO neurons.

Related to (Fig. 5d,e). a, Sagittal image of the maximum density projection showing FN^E-IO projection patterns using the fMOST approach (see Methods). A widespread distribution of FN axons is observed across different brain regions. See the tracing strategy in (Fig. 5a). b, Projection maps of four FN^E-IO single neurons. Axon projection was registered to and rendered in the Allen Mouse Brain CCF. Asterisks indicate the somata of FN^E-IO neurons (yellow structure), and the IO is marked in blue. Magenta neurons mainly project to the medulla, cerebellar cortex, and pons. Cyan neurons project to the medulla, cerebellar cortex, pons, midbrain, and thalamus. c, Comparative projection map of all FN^E-IO single neurons. The connectivity with downstream targets is represented by bars and dots on the left (ipsilateral projection) and right (contralateral projection). Colors in the dots indicate projection density, normalized to the total terminations of each cell. Detailed downstream targets are listed on the right.