Fig. 3: Excitatory nucleo-olivary pathway drives feedback CSs.
From: Excitatory nucleo-olivary pathway shapes cerebellar outputs for motor control
a, Schematics illustrating viral injection and PC recording while photoactivating FN axons in the mcMAO. Excitatory and inhibitory projections are denoted as + and −, respectively. b, Histological images showing left, ChrimsonR (red) expression in the FN neurons and DiO-labeled recording track (green) in the posterior vermis (1 of 6 mice displayed) and right, optic fiber tip over the mcMAO. c, Example PC recording (asterisk represents CS) and raster plot of CS activity following 10-ms photoactivation. d, Average PSTH (mean ± s.e.m.), probability, onset and peak timing of photoactivated CSs (n = 42 PCs from six mice). Dots represent individual neurons, and bars represent mean ± s.e.m. e, Raster plots and PSTH traces of CS activity from an example vermal PC, following 10-ms single pulse (black), 250-ms train (red, 50 Hz and 50% duty cycle) and 500-ms train (blue, 50 Hz and 50% duty cycle) stimulations. The photoactivation intensity was 3.0 mW for all protocols. f, Summary of CS activities from all PCs. Upper: average PSTHs (mean ± s.e.m.). The numbers of cells for individual conditions are shown in the panel, from six mice. Lower: timing of CS modulation following different photoactivation protocols (one-way ANOVA with Tukey’s multiple comparisons, P = 0.37 for onset and P = 0.09 for peak time). Dots represent individual neurons, and bars represent mean ± s.e.m.
